I have a Varian 1200L LC triple quad on which I need a little 'seasoned operator' advice, since Agilent no longer supports this instrument and seems to not have anyone there that has been brought on from Varian who knows the instrument, and very litttle experience in my lab with anything other than running routine samples (no real maintenance needed in the couple years it was run and then shelved before I started using it). My questions, if you have and currently run (or can recall from running it) are: When the instrument is pumped down, what is your usual manifold pressure without the CID gas on? I'm seeing 4E-6 Torr with a 40 C manifold temperature, which I think is fine (the hardware manual says to wait to operate the instrument after reaching 5 E-6). What speed does your turbo pump run at, and how long does it take to spin up from 0 to 90%? My turbo runs at 97-98%, and I've never seen it run at 100%.
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If I try to set the manifold temperature above 50 C, the turbo pump speed drops from about 97-98% at 40C to about 85% or lower, and I can't get the manifold temperature to rise above 54-55 C no matter what setpoint I enter (max 65 C). At 55 C, the vacuum pressure rises to 6.5 to 6.7E-6 Torr and I start to see a pressure reading in the CID gas (Q2) region of about 0.15-0.2 mTorr. Is this the usual situation with this instrument, or is this indicative of a vacuum (or other) problem? Is there any benefit to running hotter or colder than the recommended 42 C?
Is there an optimal temperature for the housing? I'm running ESI, and I have the option to run at 18-65C, but I don't know if there's an optimum (besides the recommended 50 C in the hardware manual). Any tips/tricks/advice is appreciated! Out of interest, what is the serial number of this instrument? What version of workstation are you running? Are you actually observing any issues with the system?
What country are you in? The normal specs for the turbo are 100% (+/- 1%), so you are just outside of this range. Peugeot 307 Workshop Manual more.
My only concern may be that your turbo is overheating slightly, so by increasing the manifold temperature you are causing the turbo to heat even more. Some have a safety feature to slow the turbo down if it's over heating, so you may be seeing this. You might be able to click on the turbo 'picture' and find out it's temperature depending on the model and software you are running. As for temperatures, the manifold temperature only really effects the RF stability so I would leave at 42. The heaters are only small, so might not be able to heat to maximum. The API housing is defaulted to 50 deg and I've never seen anyone use anything different, so stick with this. I'm running Varian MS Workstation 6.9, service pack 1.